Virasign is a viral taxonomic classification tool designed for nanopore sequencing data.
Virasign (Viral Read ASSIGNment) is a viral taxonomic classification and reference selection tool for nanopore data. It maps long-read sequencing data (via minimap2) against viral databases (RVDB, RefSeq, or custom accessions) and performs taxonomic classification to identify viral species. Virasign generates comprehensive interactive HTML reports with filterable tables, charts and heatmaps. For each identified virus, Virasign also provides the closest reference sequence, mapped reads in FASTQ format, and BAM files which can be used to easily generate a consensus genome and visualize data (e.g., IGV). Virasign includes options to blind yourself from certain incidental findings (such as HIV, Hepatitis viruses, HTLV, EBV, CMV, HPV) when wanted, ensuring these findings do not appear in any output files, in line with consent guidelines and ethical research practices.