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proteomiqon-labelfreeproteinquantification

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The tool LabelFreeProteinQuantification estimates protein abundances using quantified peptide ions.

Installation

To install this package, run one of the following:

Conda
$conda install bioconda::proteomiqon-labelfreeproteinquantification

Usage Tracking

0.0.3
0.0.1
2 / 8 versions selected
Downloads (Last 6 months): 0

Description

After quantification and protein inference are performed, it is known which peptide originated from which protein, as well as the intensity of each peptide. The information available for each peptide now needs to be aggragated for their proteins. This tool performs the aggregation from the peptides to the protein in several steps. The first two aggregation steps are optional. One of them is the aggregation based on charge state. Peptides with the same sequence and modifications, but different charge states are being aggregated. The next optional step does the same for peptides with the same sequence, but different modifications. Those steps build upon each other. The last step is the aggregation of all peptides of a protein. The result of each aggregation step is given as a tab separated file. The aggregation is performed according to the given parameters for each step. If an optional aggregation is not performed, the next step takes the result from the prior aggregation. For example, if aggregation by charge and modification are skipped, the protein aggregation is performed on previously unaggregated peptides, where a peptidesequence can occur with different charge states and modifications.

About

Summary

The tool LabelFreeProteinQuantification estimates protein abundances using quantified peptide ions.

Last Updated

Feb 16, 2023 at 21:28

License

MIT

Total Downloads

4.6K

Supported Platforms

noarch