FASTX::Abi - Read Sanger trace file (.ab1 chromatograms) in FASTQ format. For traces called with hetero option, the ambiguities will be split into two sequences to allow usage from NGS tools that usually do not understand IUPAC ambiguities.
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This module reads a Sanger trace (chromatogram). If the chromatogram was saved in "hetero" mode, that is allowing IUPAC ambiguities in the basecalling, the module will return two (unphased) sequences in standard "ACGT" alphabet.
Summary
FASTX::Abi - Read Sanger trace file (.ab1 chromatograms) in FASTQ format. For traces called with hetero option, the ambiguities will be split into two sequences to allow usage from NGS tools that usually do not understand IUPAC ambiguities.
Last Updated
Nov 11, 2021 at 21:07
License
MIT
Total Downloads
11.4K
Supported Platforms
Documentation
https://metacpan.org/pod/FASTX::Abi