MitoScape: A tool for mitochondrial DNA analysis using Apache Spark.
This MitoScape version can be executed with the command:
mitoscape \
--threads <number of threads> \
--prob <classification probability> \
--ld <path to mitomap.ld file> \
--numt <path to NUMTs_hg38.txt file> \
--classifier <path to MTClassifierModel.RF folder> \
--prefix <prefix, see below for explanation> \
--out <output prefix for bam file>
mitomap.ld
, NUMTs_hg38.txt
, and MTClassifierModel.RF
are provided in the package in the $MITOSCAPE_DATA
folder.
The prefix
matches the prefix of the bam filenames generated as explained
here.
MitoScape will look for _MT_MD.bam
and _NT.bam
.