Zone Equilisation Normalisation (ZEN) is a method for normalising bigWigs of genomic signal, such as ATAC-seq, ChIP-seq and TT-seq. ZEN is avaliable within the Python package ZEN-norm, which also includes modules for reversing prior bigWig normalisation and creating plots to compare performance of normalisation methods genome-wide. For documentation, see https://github.com/Genome-Function-Initiative-Oxford/Zone-Equalisation-Normalisation.