MiXCR is a universal software for fast and accurate analysis of raw T- or B- cell receptor repertoire sequencing data. It works with any kind of sequencing data:
- Bulk repertoire sequencing data with or without UMIs
- Single cell sequencing data including but not limited to 10x Genomics protocols
- RNA-Seq or any other kind of fragmented/shotgun data which may contain just a tiny fraction of target sequences
- and any other kind of sequencing data containing TCRs or BCRs
Powerful downstream analysis tools allow to obtain vector plots and tabular results for multiple measures. Key features include:
- Ability to group samples by metadata values and compare repertoire features between groups
- Comprehensive repertoire normalization and filtering
- Statistical significance tests with proper p-value adjustment
- Repertoire overlap analysis
- Vector plots output (.svg / .pdf)
- Tabular outputs
Other key features:
- Clonotype assembly by arbitrary gene feature, including full-length variable region
- PCR / Sequencing error correction with or without aid of UMI or Cell barcodes
- Robust and dedicated aligner algorithms for maximum extration with zero false-positive rate
- Supports any custom barcode sequences architecture (UMI / Cell)
- Human, Mice, Rat, Spalax, Alpaca, Monkey
- Support IMGT reference
- Barcodes error-correction
- Adapter trimming
- Optional CDR3 reconstruction by assembling overlapping fragmented sequencing reads into complete CDR3-containing contigs when the read position is floating (e.g. shotgun-sequencing, RNA-Seq etc.)
- Optional contig assembly to build longest possible TCR/IG sequence from available data (with or without aid of UMI or Cell barcodes)
- Comprehensive quality control reports provided at all the steps of the pipeline
- Regions not covered by the data may be imputed from germline
- Exhaustive output information for clonotypes and alignments:
- nucleotide and amino acid sequences of all immunologically relevant regions (FR1, CDR1, ..., CDR3, etc..)
- identified V, D, J, C genes
- comprehensive information on nucleotide and amino acid mutations
- positions of all immunologically relevant points in output sequences
- and many more informative columns
- Ability to backtrack fate of each raw sequencing read through the whole pipeline
License
Copyright (c) 2014-2022, MiLaboratories Inc. All Rights Reserved
Before downloading or accessing the software, please read carefully the
License Agreement available at:
https://github.com/milaboratory/mixcr/blob/develop/LICENSE
By downloading or accessing the software, you accept and agree to be bound
by the terms of the License Agreement. If you do not want to agree to the terms
of the Licensing Agreement, you must not download or access the software.
